The use of biofertilizer is low cost when compared to chemical fertilizer Pseudomonas fluorescens are the most important components of biofertilizer. Bio-fertilizers "eco-friendly” fertilizers. Biofertilizers are the substances which contain living microorganism which when applied to the plant helps then to grown without causing any nutrient loss of soil and pollution. The application of biofertilizer is cheaper than the inorganic. The biofertilizer do not cause damage to the soil health and natural environment. Thus the present study focused on the mass production of bio-fertilizer from Pseudomonas fluorescens for its large scale application. DF media was used for the mass production of bio-fertilizer.