In Mexico, there are several types of wild and criollo avocados that constitute a genetic heritage of this species; these avocados currently grow in an unordered manner on farmer's lands and in backyards, and they need to be studied as they are being lost because of agricultural activities and edaphoclimatic and phytosanitary factors. On the other hand, in orchards and avocado nurseries, high amounts of chemical fertilizers and pesticides are used affecting the physicochemical and microbiological properties of the soil, modifying the flora and fauna and polluting aquifers and springs that cause health problems among consumers. Therefore, it is very important to have a more friendly agriculture with the nature. The aim of this work was to evaluate under nursery conditions, the effect of organic fertilizers on 12 genotypes (rootstocks) of native avocados of Ometepec, Guerrero, Mexico, under an experimental design of random blocks, with four treatments: T1: sheep manure,T2: Bovine manure, T3: mycorrhizae and T4 (control: water) in four replicates. The variables were: plant height (PH), stem diameter (SD), number of leaves: young (NYL) and mature (NML) per plant; and the content of NO3-, K+, Ca2+ and Na+ ions obtained by petiole extraction, and the chlorophyll content measured with SPAD, in young (CYL) and mature (CML) leaves. Additionally, an analysis of variance and Tukey mean tests (P ≤ 0.01 and 0.05) and LSD (P ≤ 0.05) were done. It was found that sheep manure was superior to other treatments in PH (76.7 cm), SD (7.2 mm), NYL (6.5 leaves/plant), NML (18.4 leaves/plant), CML (40.2 SPAD) and Ca2+(1495ppm). In conclusion, the sheep manure was better than the bovine, mycorrhiza and control (water) as it affected positively the behavior of rootstocks in plant height, stem diameter and number of young and mature leaves. In addition, organic nutrition showed no significant response in the chlorophyll content of young and mature leaves. Young leaves only reached 50% of the chlorophyll content compared to mature leaves.