This research was aimed the composition of the substrate, inoculum dose and fermentation time suitable for the growth of Pleurotus ostreatus on cocoa pod substrate mixtures with different nitrogen sources (tofu waste, soy milk waste and rice bran) of the cellulase enzyme activity, laccase enzyme activity, crude fiber decreased, and crude fiber digestibility. This study used an experimental method with a completely randomized design (RAL) 3x3x3 factorial with 2 replications. Factor A is a substrate composition with a ratio of 80% cocoa pod and 20% for tofu waste, soy milk waste, and rice bran. Factor B is an inoculum dose of 6%, 8% and 10% with Factor C is the fermentation time of 7, 9 and 11 days. The results of the analysis showed that an interaction of the substrate composition and fermentation time with Pleurotus ostreatus which had a significant effect (P <0.05) of the activity of cellulase enzymes, laccase enzyme activity, crude fiber decreased and crude fiber digestion. The results of the study can be concluded that the selected treatment was found in the composition of 80% cocoa pod and 20% tofu waste with 9 days fermentation time (A1C2). In this condition cellulase enzyme activity was obtained of 2.70 U/ml, the laccase enzyme activity of 12.33 U/ml, crude fiber decreased of 44.01% and crude fiber digestibility of 53.78%.

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