DTIC AD1015187: Differences between Human and Rodent Plasmacytoid Dendritic Cells May Explain the Pathogenic Disparity of Hantavirus Infection

by: Defense Technical Information Center

Publication date: 2016-06-01

Topics: DTIC Archive, Lombardi,Vincent C, Whitemore Peterson Institute for Neuro-Immune Disease Reno United States, bunyaviridae infections, Infectious diseases, TRANSCRIPTION FACTORS, Transcription(Genetics), mice,

Collection: dticarchive; additional_collections

Language: English

Unlike asymptomatic infection in rodents, hantaviruses cause two acute febrile diseases in humans: hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardio-pulmonary syndrome (HCPS). The purpose of this project is to evaluate the ability of hantaviruses to infect human and murine plasmacytoid dendritic cells (the primary circulating producers of type I interferon) and to evaluate the role of pDC granzyme in the pathogenesis of hantavirus infection. Our overreaching hypothesis is that the disparity between the asymptomatic rodent infection and the pathological human infection is a result of differences between how these cells respond to infection. The scope of this work will primarily encompass transcriptional analysis of pDCs isolated from human and mice using RNAseq and confirmed by quantative PCR.